Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,151,934	G→T	L397I (CTT→ATT)	yegI ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,151,934	0	G	T	100.0%	157.6 / NA	58	L397I (CTT→ATT)	yegI	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (58/0);  total (58/0)

AGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGC  >  W3110S.gb/2151906‑2151963                             |                              agcGATAACTCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:495910/1‑58 (MQ=255) agcGATAACGCTGACTGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1146957/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTcg               >  1:1105617/1‑45 (MQ=38) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAAc      >  1:500353/1‑54 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAgg   >  1:456241/1‑57 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAgg   >  1:457841/1‑57 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAgg   >  1:77312/1‑57 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:56952/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:625175/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:556432/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:519627/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:496764/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:488317/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:463526/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:457174/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:441418/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:395399/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:62200/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:322535/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:631717/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:647489/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:688998/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:74613/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:771109/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:824607/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:84456/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:887226/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:925898/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:973640/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:981956/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:134913/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1077683/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1081818/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:108464/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1087992/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1106534/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1110657/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:11316/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1185545/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1205190/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:12235/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1238938/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1267517/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1339462/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:366680/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1356642/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1417549/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1435196/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1445673/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1446191/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1461277/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:148185/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:153131/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:158688/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:237881/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:269075/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:279741/1‑58 (MQ=255) agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc  >  1:1011740/1‑58 (MQ=255)                             |                              AGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGC  >  W3110S.gb/2151906‑2151963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑