Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,215,682	A→G	I205I (ATT→ATC)	yehT ←	predicted response regulator in two‑component system withYehU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,215,682	0	A	G	100.0%	139.6 / NA	51	I205I (ATT→ATC)	yehT	predicted response regulator in two‑component system withYehU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/51);  total (0/51)

ACGAATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAGTGGTG  >  W3110S.gb/2215679‑2215740    |                                                           aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTTACGATGGCAGCGCAGTAgtgggg  <  1:1200549/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:712893/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:288138/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:325800/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:349384/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:460346/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:466857/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:506039/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:555829/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:560523/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:584967/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:611592/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:635651/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:636962/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:259712/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:739911/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:755719/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:756529/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:768161/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:780044/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:835299/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:841027/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:882548/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:887400/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:903611/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:919707/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1276526/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1014508/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:109487/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1101240/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1118300/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:113425/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1136192/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1137961/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1139425/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1157970/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1173927/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1232442/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:262754/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1289310/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1320570/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1423241/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:142798/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1467814/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1469843/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:16998/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:171259/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:234094/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:252879/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:1011964/62‑3 (MQ=255) aCGGATCTCCTGTAAATGCACGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAgtgggg  <  1:112248/62‑3 (MQ=255)    |                                                           ACGAATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGACGATGGCAGCGCAGTAGTGGTG  >  W3110S.gb/2215679‑2215740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑