Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,226,766	A→G	E416E (GAA→GAG)	dld →	D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,226,766	0	A	G	100.0%	178.2 / NA	64	E416E (GAA→GAG)	dld	D‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/64);  total (0/64)

GCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGC  >  W3110S.gb/2226728‑2226788                                       |                       gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGGCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1276418/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTActc  <  1:1250321/61‑3 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:450934/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:568568/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:54172/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:540354/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:53495/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:50549/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:493970/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:619672/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:449218/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:441390/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:433178/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:404249/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:392550/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:378901/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:377623/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:341806/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:615757/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:335617/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:624022/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:760146/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:760522/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:7711/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:803327/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:829449/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:832304/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:852243/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:852253/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:881630/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:954773/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:955602/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:96322/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1377707/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1037978/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1096532/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1107798/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1131678/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1163543/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1189047/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1205607/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1244913/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1291230/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1294972/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1303083/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1355139/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:100024/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:187625/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:326460/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:306749/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:298173/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:250471/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:247695/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:24221/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1406840/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:149190/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1446959/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1436256/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1427361/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1423647/61‑1 (MQ=255) gCCAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1410924/61‑1 (MQ=255)  ccAAATCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:802299/60‑1 (MQ=255)      aTCGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1079707/56‑1 (MQ=255)        cGTGGCTAGTGGATTATTTCAAACAGGCCGAGGGCGATTTCTTTGTCTGTACGc  <  1:1108955/54‑1 (MQ=255)                                       |                       GCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGC  >  W3110S.gb/2226728‑2226788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑