| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,253,524 | G→A | A158A (GCG→GCA) | yeiH → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,253,524 | 0 | G | A | 99.1% | 308.1 / ‑5.6 | 113 | A158A (GCG→GCA) | yeiH | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (0/112); total (1/112) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.85e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATC > W3110S.gb/2253500‑2253566 | ggCGTCTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACGgtt < 1:128218/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:440092/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:596151/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:593873/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:59146/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:548049/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:524942/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:518458/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:508061/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:500637/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:498983/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:483548/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:466275/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:604791/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:40589/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:399449/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:394778/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:383793/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:342637/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:342391/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:326992/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:300293/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:294234/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:26148/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1002524/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:847992/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:988196/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:947010/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:924962/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:921926/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:921056/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:897165/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:881942/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:871316/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:865093/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:863958/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:862609/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:247267/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:842352/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:804313/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:795520/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:7675/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:758941/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:758201/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:723069/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:702691/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:683687/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:665389/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:656125/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1128911/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:243238/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1239902/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1230522/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1226031/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1180981/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1174958/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1174840/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1159212/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1153840/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1151425/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1148399/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1244742/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1121888/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1113057/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1081042/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1058361/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:102766/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1026488/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1024700/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1022911/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1021698/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1019713/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1019524/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1256333/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:211156/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:202128/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:200706/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:190909/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:183791/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:148142/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1467346/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:145528/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1423461/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1387336/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1377038/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1371555/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1330957/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1319496/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1312190/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1297257/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1295965/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:127695/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1264960/61‑1 (MQ=255)ggCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1251048/61‑1 (MQ=255) gCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:879749/60‑1 (MQ=255) gCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1237912/60‑1 (MQ=255) gCGACTGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:96972/60‑1 (MQ=255) cTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATc > 1:1278339/1‑62 (MQ=255) tGAGCCGGTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:424208/55‑1 (MQ=255) gTAGTGAAAGCAGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgtt < 1:1256517/48‑1 (MQ=255) | GGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATC > W3110S.gb/2253500‑2253566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |