Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,276,852	A→G	T385T (ACA→ACG)	yejA →	predicted oligopeptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,276,852	0	A	G	100.0%	86.6 / NA	32	T385T (ACA→ACG)	yejA	predicted oligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/32);  total (0/32)

GTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGC  >  W3110S.gb/2276837‑2276882                |                               gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGTTATCCAAAGGc  <  1:947020/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:188636/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:965108/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:872763/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:852184/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:795973/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:670917/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:572068/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:550617/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:444624/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:244563/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:230660/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:224560/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:192773/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:191897/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1025700/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:165847/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1446587/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:143902/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1374617/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:137257/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:136034/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1347558/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1238885/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1232089/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1218739/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1213697/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1169427/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1120389/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1099241/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAATCTACCAGCCGCCGGTATCCAAAGGc  <  1:1026562/44‑1 (MQ=255) gccAGAAGTCTTCACGCAAAACTACCAGCCGCCGGTATCCAAAGGc  <  1:466286/44‑1 (MQ=255)                |                               GTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGC  >  W3110S.gb/2276837‑2276882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑