Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,278,301 G→A A263A (GCG→GCA)  yejB → predicted oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,278,3010GA99.4% 453.7 / NA 165A263A (GCG→GCA) yejBpredicted oligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (163/0);  minor base C (1/0);  total (165/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

AGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTA  >  W3110S.gb/2278282‑2278316
                   |               
agcaaTACGTGGTGACGGCTCGTGCGAAAGGGGTa  >  1:645494/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:491247/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:641044/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:615203/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:610636/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:605948/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:578900/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:572352/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:563153/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:549951/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:54074/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:503842/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:494840/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1009/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:45468/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:436712/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:404126/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:37556/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:366260/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:347025/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:326155/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:320113/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:312207/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:303667/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:302114/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:287152/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:875064/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:990060/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:96335/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:960139/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:954233/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:948720/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:936088/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:92391/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:901988/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:898613/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:894701/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:880028/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:658028/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:84890/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:839972/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:830914/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:816794/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:81038/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:77219/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:754582/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:724351/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:710404/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:702844/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:674158/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1173812/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:278174/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:128387/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1283450/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1270748/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1265501/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1246551/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1229355/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1224417/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1220150/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1215382/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1214266/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1197970/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1294191/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1172644/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1150923/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1139108/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1095697/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1065947/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1055299/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1038819/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1029324/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1021131/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1019408/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1015383/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1435617/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:27472/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:264343/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:258124/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:256362/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:229561/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:20416/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:194051/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:155827/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:149357/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1467871/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:145970/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1456453/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1299716/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1413595/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1392073/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1381023/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1366345/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1364830/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1343419/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1332568/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1328264/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1321476/1‑35 (MQ=37)
agcaaTACGTGGTGACGGCACGTGCGAAAGGGGTa  >  1:1303380/1‑35 (MQ=37)
agcaaTACGTGGAGACGGCACGTGCTAAAGGGGTa  >  1:828005/1‑35 (MQ=255)
                   |               
AGCAATACGTGGTGACCGCGCGTGCGAAAGGGGTA  >  W3110S.gb/2278282‑2278316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑