| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 222,052 | G→A | T198T (ACC→ACT) | metN ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 222,052 | 0 | G | A | 86.5% | 368.0 / 33.9 | 178 | T198T (ACC→ACT) | metN | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/24); new base A (154/0); total (154/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.07e-30 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 178 total aligned reads displayed.
TGATGACCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCG > W3110S.gb/222003‑222106 | tgatgaCCGCCTCGCAATCACAAATGCGCTTCACAACGACCATTTCGTGAGTGATCAACAGa > 1:990628/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCGCAACGTCCATTTCGTGAGTGATCAACAGa > 1:224526/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAg > 1:288325/1‑61 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAg > 1:933718/1‑61 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAg > 1:646513/1‑61 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAg > 1:350171/1‑61 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAg > 1:1384046/1‑61 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAg > 1:1316353/1‑61 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:492007/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1038868/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:638462/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:615347/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:60229/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:590718/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:583696/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:558131/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:535382/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:520216/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:508894/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:656375/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:491041/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:442486/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:419729/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:406898/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:400095/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:369157/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:110995/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1044634/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:317195/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:84928/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:100724/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:969981/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:952015/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1016930/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:931958/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:921823/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:913779/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:903265/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:875235/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:868846/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:360027/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:838565/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:825900/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:808569/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:787997/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:756602/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:74032/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:715214/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:690040/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:664626/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1301436/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:150532/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1444706/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:140988/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1004414/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1396125/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1359977/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1355235/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1346154/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1324794/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1316870/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1406035/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1275056/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1240780/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1238779/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1198443/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1189343/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1155141/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1132702/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:1123565/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:112/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:15824/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:283816/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:278197/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:261666/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:255231/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:254757/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:105268/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:212526/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:194274/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:188624/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:179279/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:17033/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:165015/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGAGTGATCAACAGa > 1:158754/1‑62 (MQ=255)tgatgaCCGCCACGCAATCACAAATGCGCTTCACAACATCCATTTCGTGAGTGATCAACAGa > 1:374830/1‑62 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:295089/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:737415/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:536629/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:1142222/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:535844/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:1172390/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:1195273/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:386968/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:1097911/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:1373996/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:1104278/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:460996/62‑1 (MQ=255) tttCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:751876/62‑1 (MQ=255) tCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCg < 1:153345/60‑1 (MQ=255) | TGATGACCGCCACGCAATCACAAATGCGCTTCACAACGTCCATTTCGTGGGTGATCAACAGAATCGTCAACCCCAGACGGCGGTTGATGTCTTTCAGCAGTTCG > W3110S.gb/222003‑222106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |