Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,293,655	T→C	intergenic (‑240/‑179)	insH ← / → narP	IS5 transposase and trans‑activator/DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,293,655	0	T	C	96.7%	75.3 / ‑6.4	30	intergenic (‑240/‑179)	insH/narP	IS5 transposase and trans‑activator/DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (29/0);  minor base A (1/0);  total (30/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCTTGATGAGAA  >  W3110S.gb/2293604‑2293664                                                    |          aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:325001/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:96915/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:957306/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:824839/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:778179/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:776424/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:763992/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:667767/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:631221/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:613159/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:507126/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:489192/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:468428/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:361227/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1020940/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:235780/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1441955/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1439648/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1397757/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1373637/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1329440/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1263146/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1179650/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1128632/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1102561/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1080924/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1055699/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg         >  1:1047635/1‑54 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCTGATgagat  >  1:890236/1‑60 (MQ=255) aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCAtg         >  1:1151687/1‑54 (MQ=255)                                                    |          ACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCTTGATGAGAA  >  W3110S.gb/2293604‑2293664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑