Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,357,574	T→A	V86V (GTT→GTA)	glpA →	sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,357,574	0	T	A	100.0%	167.0 / NA	61	V86V (GTT→GTA)	glpA	sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/61);  total (0/61)

CCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGC  >  W3110S.gb/2357547‑2357605                            |                                ccTGAAACGGATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1102304/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCAGTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:526376/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:692789/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:368344/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:394683/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:404828/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:406474/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:422349/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:455392/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:462540/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:488355/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:524181/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:541274/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:580276/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:609528/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:610966/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:672201/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:342340/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:702943/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:742101/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:748696/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:753298/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:76188/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:762265/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:775679/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:779145/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:788642/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:923695/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:934593/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:951470/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:964767/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:325544/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1041370/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:10938/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:115534/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1168536/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1206485/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1240890/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1249796/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1253054/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1293249/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1316939/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1325415/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1361653/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1371101/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1395958/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1401468/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1407266/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1444618/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1447848/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1466048/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1466728/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:195961/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:224165/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:225171/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:228466/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:237717/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:239711/59‑1 (MQ=255) ccTGAAACGCATTGCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:103126/59‑1 (MQ=255)              gCACGTCACTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:1328312/46‑1 (MQ=255)                      cTGCGTAGAACCAACCAACGGCCTGTTTATCACCCTGc  <  1:677675/38‑1 (MQ=255)                            |                                CCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGC  >  W3110S.gb/2357547‑2357605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑