| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,363,126 | C→T | A130A (GCG→GCA) | yfaU ← | predicted 2,4‑dihydroxyhept‑2‑ene‑1,7‑dioic acid aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,363,126 | 0 | C | T | 88.6% | 373.9 / 26.0 | 176 | A130A (GCG→GCA) | yfaU | predicted 2,4‑dihydroxyhept‑2‑ene‑1,7‑dioic acid aldolase |
| Reads supporting (aligned to +/- strand): ref base C (20/0); new base T (0/155); total (21/155) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-26 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01 | |||||||||||
Only 100 of 176 total aligned reads displayed.
GACAAAGCGAATCGTTAACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATA > W3110S.gb/2363074‑2363176 | gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:565154/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:70865/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:701608/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:695678/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:687420/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:668922/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:653574/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:647200/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:635977/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:618442/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:608983/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:57689/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:719350/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:549607/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:539292/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:50277/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:501653/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:499746/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:487392/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:469306/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:418562/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:390669/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1003656/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:366585/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:81767/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:992940/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:97902/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:953477/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:941347/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:933849/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:928939/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:912832/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:883392/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:870644/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:862478/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:826554/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:388519/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:814796/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:802427/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:798674/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:793329/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:789841/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:763104/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:749448/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:741438/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:739264/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:726753/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1234288/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1388293/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1359816/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1347554/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1323047/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1315341/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:130007/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1281484/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1273124/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1259906/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1248587/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:303633/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1233794/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1185914/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1178373/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1147992/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1146476/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:112346/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1120348/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:105644/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1054894/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1019650/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1411369/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:197689/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:208735/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1407125/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:183729/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:177619/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:21373/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1470721/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:251360/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1452732/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1443895/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1430860/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:351444/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCAGt < 1:1097177/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCAAGCGTGCAGCCCGt < 1:177399/62‑1 (MQ=255)gACAAAGCGAATCGTTGACTTGCGCCATGTAATTCCCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1318321/62‑1 (MQ=255) gAATCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:1131535/54‑1 (MQ=255) aTCGTTGACTTGCGCCATGTATTTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:475903/52‑1 (MQ=255) tCGTTGACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:193113/51‑1 (MQ=255) ttGCGCCATGTAATTCTCAATGCGTCCCCAGCGTGCAGCCCGt < 1:525578/43‑1 (MQ=255) cGTCCCCAGCGGGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATa > 1:443944/1‑62 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTc > 1:116469/1‑47 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGAt > 1:1211810/1‑61 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGAt > 1:378144/1‑61 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGAt > 1:100958/1‑61 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATa > 1:508347/1‑62 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATa > 1:1301919/1‑62 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATa > 1:611626/1‑62 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATa > 1:289058/1‑62 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATa > 1:1028025/1‑62 (MQ=255) cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATa > 1:1374838/1‑62 (MQ=255) | GACAAAGCGAATCGTTAACTTGCGCCATGTAATTCTCAATGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATA > W3110S.gb/2363074‑2363176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |