| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,363,339 | A→G | H59H (CAT→CAC) | yfaU ← | predicted 2,4‑dihydroxyhept‑2‑ene‑1,7‑dioic acid aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,363,339 | 0 | A | G | 83.5% | 149.0 / 16.9 | 79 | H59H (CAT→CAC) | yfaU | predicted 2,4‑dihydroxyhept‑2‑ene‑1,7‑dioic acid aldolase |
| Reads supporting (aligned to +/- strand): ref base A (13/0); new base G (0/66); total (13/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.79e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCA > W3110S.gb/2363306‑2363385 | gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:432262/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:522124/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:518038/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:487090/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:48502/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:454877/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:447510/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:438121/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:435499/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:534110/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:430519/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:420657/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:400999/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:391120/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:342322/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:341384/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:244606/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:209341/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:867510/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:996475/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:981397/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:972190/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:924969/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:913262/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:889635/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:8855/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:86934/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1025814/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:862137/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:841045/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:712959/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:700396/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:661046/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:601891/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:579295/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:551021/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1159889/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:128615/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1261670/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1261622/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1243396/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:124026/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1184099/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1181128/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1172624/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:188378/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1143273/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1135669/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1133425/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1122488/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1112068/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:104513/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1030098/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1293474/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1318900/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1320181/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1335474/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1289543/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:195238/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:188095/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:182271/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:14925/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1453162/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1445388/40‑1 (MQ=255)gtggctggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1445996/40‑1 (MQ=255)gtggcgggcATAGGGCGCTACCGCCTGTAGCTGGTGATaaa < 1:1007662/40‑1 (MQ=38) aCCGCCTGTAGCTGATGATAAAGATCCTGATTGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:923329/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGgtgt > 1:328984/1‑37 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCt > 1:1147069/1‑49 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:814569/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:1377857/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:654881/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:370062/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:162437/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:605013/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:1199028/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:600431/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:102868/1‑61 (MQ=255) aCCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCa > 1:1204758/1‑61 (MQ=255) | TTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCA > W3110S.gb/2363306‑2363385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |