| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,367,337 | G→A | L323L (CTG→TTG) | yfaY ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,367,337 | 0 | G | A | 88.6% | 218.1 / 13.6 | 105 | L323L (CTG→TTG) | yfaY | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (2/10); new base A (93/0); total (95/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTT > W3110S.gb/2367304‑2367384 | gCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGTCAAAATGGTTGGCCCGCCGTTCt < 1:261806/62‑1 (MQ=255)gCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCt < 1:641473/62‑1 (MQ=255)gCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCt < 1:5702/62‑1 (MQ=255)gCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCt < 1:792467/62‑1 (MQ=255)gCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCt < 1:1210644/62‑1 (MQ=255)gCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCt < 1:1364284/62‑1 (MQ=255)gCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCt < 1:132497/62‑1 (MQ=255) cTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGTCCGCCGTTCt < 1:659628/61‑1 (MQ=255) cTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCt < 1:1422928/61‑1 (MQ=255) cTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCt < 1:802143/61‑1 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCTGCCGTTCTGTAATCCAGTgcgc > 1:1050179/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTATTCCAGTgcgc > 1:431439/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:650062/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:538873/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:632303/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:632285/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:602769/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:655387/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:675121/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:532293/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:507426/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:45350/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:431250/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:418464/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:359659/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:353450/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:351639/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:335567/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:327741/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:31748/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:855682/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:983593/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:979574/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:946181/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:934628/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:934156/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:911893/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:91175/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:895502/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:892139/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:880073/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:870297/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:656196/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:825134/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:807958/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:796522/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:751381/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:732590/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:731552/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:719608/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:696458/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:692562/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:265564/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1112176/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1275759/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1264682/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1236278/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1235040/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1231611/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1228836/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:120544/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:118491/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1134266/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1130799/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1114396/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1296755/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1106174/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1086677/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1084121/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1065050/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:106387/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1059444/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1038473/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1038109/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1036631/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1025610/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1025339/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:184589/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:28293/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:282093/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1004423/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:234504/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:220807/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:201155/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:200886/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:195416/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:184612/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:31524/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:170995/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:168469/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:163828/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:163826/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1464130/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1424580/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1329023/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1319413/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAACCCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1312820/4‑62 (MQ=255) ccagAAACAGCCAGTGCCAAACCAGCAAAATGGTTCGCCCGCCGTTCTGTAATCCAGTgcgc > 1:1457681/4‑62 (MQ=255) aaCAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGttt > 1:24816/1‑62 (MQ=255) aaCAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGttt > 1:264882/1‑62 (MQ=255) | GCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTT > W3110S.gb/2367304‑2367384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |