Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,408,753 G→A G177G (GGC→GGT)  nuoB ← NADH:ubiquinone oxidoreductase, chain B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,408,7530GA100.0% 447.6 / NA 158G177G (GGC→GGT) nuoBNADH:ubiquinone oxidoreductase, chain B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (158/0);  total (158/0)

Only 100 of 158 total aligned reads displayed.

CAACCACCCAGGAGAGCGGACGACGTTCTTTGCCGATAGATTCCTGCAACAGCATC  >  W3110S.gb/2408722‑2408777
                               |                        
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGAt                     >  1:748546/1‑37 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCAt   >  1:781649/1‑55 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:469054/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:652194/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:645514/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:64056/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:635067/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:627145/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:56378/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:543323/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:531695/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:480763/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:47510/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:666674/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:440317/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:410364/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:408604/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:388752/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:372918/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:317991/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:304318/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:299291/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:287806/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:285420/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:268318/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1015245/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:866686/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:967359/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:960621/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:953910/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:950000/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:949946/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:939910/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:93275/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:923660/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:910625/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:90189/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:893388/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:65401/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:853344/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:849317/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:831848/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:757862/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:718873/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:717616/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:716908/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:716267/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:708548/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:700336/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:255687/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1167731/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1285038/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1282918/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1281417/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1276204/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1269957/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1269622/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1235701/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1215791/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1212378/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1185960/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1180648/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:117225/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1297784/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1151196/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1145784/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:114268/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1126782/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1121839/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1097656/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:106997/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1069964/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1041686/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1034905/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1020066/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1466782/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:226630/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:224973/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:218488/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:214793/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:205129/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:190510/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:18597/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:171728/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:167876/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:150819/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:150735/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:245624/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1445054/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1443416/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1414305/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1403756/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1374914/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1337610/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1328941/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1312620/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1312361/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1308864/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAGCATc  >  1:1304275/1‑56 (MQ=255)
cAACCACCCAGGAGAGCGGACGACGTTCTTTACCGATAGATTCCTGCAACAACATc  >  1:361455/1‑56 (MQ=255)
                               |                        
CAACCACCCAGGAGAGCGGACGACGTTCTTTGCCGATAGATTCCTGCAACAGCATC  >  W3110S.gb/2408722‑2408777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑