Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,431,027	G→A	F112F (TTC→TTT)	hisQ ←	histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,431,027	0	G	A	80.8%	168.1 / 29.3	99	F112F (TTC→TTT)	hisQ	histidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (5/14);  new base A (80/0);  total (85/14)
Fisher's exact test for biased strand distribution p-value = 3.06e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCCTTTCGGCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCGA  >  W3110S.gb/2430995‑2431064                                 |                                      tGTCCTTTCGGCACTGCCATAAACGCGCCACTAAACGTTTCGGTaaaa                        >  1:160851/1‑48 (MQ=37) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGttt                                >  1:640363/1‑40 (MQ=37) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:607944/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:636831/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:594596/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:590049/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:58960/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:563013/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:508874/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:490917/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:488447/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:486653/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:478920/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:475450/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:466001/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:446734/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:436768/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:43258/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:422266/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:420269/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:399414/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:395642/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:320681/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:8591/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:987236/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:965972/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:956966/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:953692/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:943499/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:922390/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:917603/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:868357/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:866851/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:864557/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:625908/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:855637/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:807576/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:797413/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:787430/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:782963/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:761416/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:725556/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:715037/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:674580/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1013343/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1144488/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:124391/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1228512/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1217335/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1211045/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1201336/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:119482/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1170556/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1166806/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1164910/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:278629/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1128112/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1112949/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1097277/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1085241/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1080515/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1068287/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1053512/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1038102/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1011581/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1292316/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:198820/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:217451/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:223129/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:189099/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:182413/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:249432/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:262049/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:272362/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:318180/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1293153/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:1285498/1‑48 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTCGGTaaa                         >  1:896150/1‑47 (MQ=38) tGTCCTTTCGGCACTGCCATAAACGCGCCACGAAACGTTTAGGTaaaa                        >  1:497351/1‑48 (MQ=255) tGTCCTTTCGGCACTGACATAAACGCGCCACGAAACGTTTCGGTaaaa                        >  1:534000/1‑48 (MQ=37)         cGGCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATaaaa      >  1:1216049/1‑58 (MQ=255)         cGGCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAAcc    >  1:1351324/1‑60 (MQ=255)         cGGCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAAcc    >  1:1409442/1‑60 (MQ=255)         cGGCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAAcc    >  1:744638/1‑60 (MQ=255)         cGGCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAAcc    >  1:14805/1‑60 (MQ=255)          ggCACTTCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:991401/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:1118003/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:1085695/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:1120480/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:423578/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:1351993/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:1055752/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:951431/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:1053544/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:656126/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:651900/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:493125/61‑1 (MQ=255)          ggCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:59913/61‑1 (MQ=255)           gCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCga  <  1:1126925/60‑1 (MQ=255)                                 |                                      TGTCCTTTCGGCACTGCCATAAAAGCACCACGGAACGTTTCGGTAAAATAAGCACCGTAGATAAAACCGA  >  W3110S.gb/2430995‑2431064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑