Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,450,698	G→A	Y103Y (TAC→TAT)	yfcA ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,450,698	0	G	A	100.0%	172.4 / NA	62	Y103Y (TAC→TAT)	yfcA	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (62/0);  total (62/0)

ATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACC  >  W3110S.gb/2450648‑2450707                                                   |          aTACAAATTGCGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:928036/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAGCATACTGCAcc  >  1:1336636/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCa    >  1:122608/1‑58 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:414782/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:515895/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:511518/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:510346/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:425181/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:424413/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:420252/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:529353/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:404601/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:404539/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:399519/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:394283/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:373206/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:372507/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:527594/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:35250/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:539504/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:655932/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:681889/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:720894/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:788868/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:855714/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:885105/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:888861/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:913227/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:930245/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:939381/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:960346/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:333105/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1044773/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1117739/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1137698/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1155940/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1172285/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1173261/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1187677/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1193662/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1267468/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:129754/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1314107/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1322445/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1326826/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1023800/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1357811/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1379755/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:30918/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1388017/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1388958/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:269462/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1441494/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:193002/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:234725/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:237703/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:241243/1‑60 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAca  >  1:600241/1‑59 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAc   >  1:1218652/1‑59 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAc   >  1:1350834/1‑59 (MQ=255) aTACAAATTACGAGGATCGGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAc   >  1:494756/1‑59 (MQ=255) aTACAAATTACGAGGATCAGTAAAATCTGCCGTAAGACATCGGCCTGAACATACTGCAcc  >  1:1337939/1‑60 (MQ=255)                                                   |          ATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACC  >  W3110S.gb/2450648‑2450707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑