Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,460,220	C→T	A76A (GCG→GCA)	yfcU ←	predicted export usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,460,220	0	C	T	100.0%	62.0 / NA	24	A76A (GCG→GCA)	yfcU	predicted export usher protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/0);  total (24/0)

GCATACCAGTAAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAT  >  W3110S.gb/2460194‑2460254                           |                                   gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGtttat                    >  1:520767/1‑43 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:320526/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:967236/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:939898/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:864693/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:843656/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:764482/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:735089/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:684658/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:656456/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:592196/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:537863/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:1093251/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:264935/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:257631/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:1456491/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:1455090/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:1412947/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:134776/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:1275830/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:1254080/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:1181378/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt  >  1:1172889/1‑61 (MQ=255) gCATACCAGTAAATATCGTACTCTTCTGCCAATGGCTATTTATTTAGTTGAACCTGTAAAt  >  1:271991/1‑61 (MQ=255)                           |                                   GCATACCAGTAAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAT  >  W3110S.gb/2460194‑2460254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑