Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,511,905	C→A	E58D (GAG→GAT)	ypdF ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,511,905	0	C	A	98.4%	165.9 / ‑5.3	63	E58D (GAG→GAT)	ypdF	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (0/62);  minor base G (0/1);  total (0/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GGTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCACCTCAACGTAATAGCGCGAATCCACCAGAATGTGCGC  >  W3110S.gb/2511845‑2511940                                                             |                                    ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTTGTAGCCTTGCGCACGGTCTTCCAcc                                     <  1:822007/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:944916/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:397016/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:309696/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:185473/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:164759/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1470460/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1433478/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:536636/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1310191/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1301712/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:681928/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1250882/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:709528/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:740420/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:756520/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1096495/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:809308/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1133064/61‑1 (MQ=255) ggTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1125185/61‑1 (MQ=255)  gTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCAcc                                     <  1:1370867/60‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACGTCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:366956/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCGGCTTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:3024/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:512319/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:900912/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:913246/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:511618/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:45757/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:928422/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:444874/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:44040/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:43637/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:427821/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:420358/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:315219/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:521914/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:902317/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:756522/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:536471/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:891711/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:564827/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:629852/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:655196/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:886732/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:708900/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:809369/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:71743/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:806103/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:747681/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1175347/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1247699/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1244484/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1234887/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:122518/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:12156/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1204294/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1195210/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1190312/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1269256/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1141830/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1134913/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1122615/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1119074/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:109696/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1083221/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1078450/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1061282/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:15594/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:377252/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:35355/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:326970/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:322380/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1018483/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:277006/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:255873/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:161511/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:403502/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1424246/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1405962/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1404569/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1367799/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1297807/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcgc  <  1:1296034/62‑1 (MQ=255)                                   tGGTAACCTTGCGTGCGGGCTTCAACATCCGCGTAATAGCGCGAATCCACCAGAATGTgcga  <  1:549751/62‑2 (MQ=255)                                                             |                                    GGTAAGCGTGTTCGTCGCGTCAAGCAAATGCAGCTGGTAGCCTTGCGCACGGGCTTCCACCTCAACGTAATAGCGCGAATCCACCAGAATGTGCGC  >  W3110S.gb/2511845‑2511940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑