| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,583,855 | G→A | V109V (GTC→GTT) | aegA ← | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,583,855 | 0 | G | A | 92.7% | 259.2 / 5.7 | 109 | V109V (GTC→GTT) | aegA | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base A (101/0); total (109/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTACCAAAAGGACAGGCC > W3110S.gb/2583831‑2583897 | cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGt > 1:732203/1‑51 (MQ=255)cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGt > 1:1178031/1‑51 (MQ=255)cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa > 1:516263/1‑52 (MQ=255)cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa > 1:239119/1‑52 (MQ=255)cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa > 1:571551/1‑52 (MQ=255)cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa > 1:73264/1‑52 (MQ=255)cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa > 1:848294/1‑52 (MQ=255)cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa > 1:863613/1‑52 (MQ=255) cttttacttttaCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:43414/1‑62 (MQ=255) cttttacttttaCTACCGCAACCGGTGTAAGAACGCTTTGCATCGTACCAAATGGACAGGcc > 1:1032604/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTAcc > 1:1012801/1‑49 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATgg > 1:420429/1‑55 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:957974/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:586439/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:573135/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:916027/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:537298/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:94106/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:532429/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:531399/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:526045/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:522429/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:660660/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:508243/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:508124/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:505755/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:496058/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:464182/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:442465/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:969058/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:400862/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:805548/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:854405/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:875387/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:876024/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:838193/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:836065/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:828691/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:821674/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:809929/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:808538/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:867455/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:800517/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:797036/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:78501/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:883905/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:894208/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:715845/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:677070/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:674290/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:666431/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1212296/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:400329/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1401878/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1396185/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1388554/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1309636/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1309144/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1300109/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1296792/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1284921/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1255767/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1254798/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1414623/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1199500/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1166420/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1147839/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1144065/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1130679/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1092165/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1081695/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1053813/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1038361/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1024287/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:268697/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:362296/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:360803/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:360568/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:355322/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:314296/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:312402/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:284719/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:274880/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:270475/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1444305/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:255499/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:241262/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:238572/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:230546/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:191246/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:182065/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:16451/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:147945/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1458094/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:1445963/1‑62 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGc > 1:1080037/1‑61 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGc > 1:693699/1‑61 (MQ=255) cTTTTACTTTTCCTACCGCAACGGGTGTCAGAAAGATTTGCATCGTACCAAATGGACAGGcc > 1:919496/1‑62 (MQ=255) cTTTTACATTTCCTACCGCAACGGGTGTCAGAACg > 1:1152634/1‑35 (MQ=19) cTTTCACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc > 1:605768/1‑62 (MQ=255) | CGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTACCAAAAGGACAGGCC > W3110S.gb/2583831‑2583897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |