Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,596,421	C→T	intergenic (‑147/+66)	purC ← / ← nlpB	phosphoribosylaminoimidazole‑succinocarboxamide synthetase/lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,596,421	0	C	T	100.0%	269.4 / NA	96	intergenic (‑147/+66)	purC/nlpB	phosphoribosylaminoimidazole‑succinocarboxamide synthetase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/96);  total (0/96)

CCGCTAAAATCGCGTTTTCCTGCAGACGCACACGTTTGCGTATCATATCAGAA  >  W3110S.gb/2596403‑2596455                   |                                   ccGTTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATGAGaa  <  1:1293552/53‑1 (MQ=255) ccGCTAAAATCGCTTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1214667/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTTTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:673639/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:456889/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:611631/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:583861/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:576539/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:548817/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:544626/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:511984/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:495670/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:483138/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:480228/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:26357/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:438858/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:364367/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:345443/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:340129/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:334421/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:318338/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:313325/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:301568/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:30156/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:292078/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:291799/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:241796/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:770094/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:997529/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:995399/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:955837/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:905437/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:903273/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:880317/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:86766/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:798879/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:789561/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:78669/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:620789/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:765431/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:756078/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:74939/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:737297/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:723715/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:721995/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:705133/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:663017/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:645382/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:625219/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1168817/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1008176/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1293173/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1281793/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1239725/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1237287/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:123664/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1210792/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1196157/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1188537/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1186919/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1175019/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1296646/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1160002/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1148683/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1144481/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1143738/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1091250/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1091035/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1068340/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1045896/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1019517/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:10123/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:139523/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:240914/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:208225/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:201396/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:197083/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:192711/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:162684/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:149776/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1455333/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1402619/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1400006/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1392701/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1382018/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:13748/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:13655/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1365152/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1340234/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1331559/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1326963/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1322100/53‑1 (MQ=255) ccGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATAATATCAGaa  <  1:1310167/53‑1 (MQ=255) ccGCTAAAATCGCGGTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:330981/53‑1 (MQ=255)  cGCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:93167/52‑1 (MQ=255)  agCTAAAATCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1243084/51‑1 (MQ=255)      aaaaTCGCGTTTTTCTGTCGACGCACACGTTTGCGTATCATATCAGaa  <  1:1312377/48‑1 (MQ=255)                   |                                   CCGCTAAAATCGCGTTTTCCTGCAGACGCACACGTTTGCGTATCATATCAGAA  >  W3110S.gb/2596403‑2596455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑