Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,614,421 A→G L39L (CTT→CTC)  yfgO ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,614,4210AG99.2% 362.1 / NA 131L39L (CTT→CTC) yfgOpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (0/130);  minor base T (0/1);  total (0/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

ACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATA  >  W3110S.gb/2614407‑2614469
              |                                                
accaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCaaata   <  1:294406/62‑3 (MQ=255)
 ccaGCAATGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:7515/62‑1 (MQ=255)
 ccaGCAACGGCGCTAGTAGGCCGCTAAAGAAAAAGATAATGCCAACACCAGCAACCAAACTa  <  1:177313/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:498375/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:610236/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:609823/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:60309/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:58908/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:58662/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:546522/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:534876/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:52905/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:52023/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:507581/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:503804/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:611525/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:480734/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:455596/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:429199/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:422024/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:420385/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:415547/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:393303/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:374815/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:362379/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:341257/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:73495/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:933339/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:893576/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:876449/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:844866/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:838436/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:83433/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:819455/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:798036/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:781909/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:772452/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:766487/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:335670/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:73167/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:719717/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:700036/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:696712/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:693705/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:684683/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:68122/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:680818/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:658493/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:653682/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:647849/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1199742/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1378051/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1350993/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1012370/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1333897/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1326194/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1325757/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1308583/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1299368/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1269153/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1232725/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1212276/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1349748/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1196199/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1172445/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1155152/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:112507/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1103186/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1075349/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:107460/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1073656/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1060578/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1012949/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:203990/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:326445/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:315568/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:295727/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:259795/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:256837/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:254179/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:252863/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:247687/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:213925/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1388506/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:202732/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:173272/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:155835/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:149788/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1461037/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1428753/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1424535/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1423746/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1402605/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1390477/62‑1 (MQ=255)
 ccaGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCAAAAACCAGCAACCAAAATa  <  1:1130115/62‑1 (MQ=255)
  caGGAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:744269/61‑1 (MQ=255)
  caGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:482676/61‑1 (MQ=255)
  caGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1139409/61‑1 (MQ=255)
  caGCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1053103/61‑1 (MQ=255)
    gCAACGGCGCGAGTAGGCCGCTAAAGAAAAAGATAATGCCAAAACCAGCAACCAAAATa  <  1:1345603/59‑1 (MQ=255)
              |                                                
ACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATA  >  W3110S.gb/2614407‑2614469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑