Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,675,556	C→A	A488S (GCT→TCT)	yphE ←	fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,675,556	0	C	A	95.6%	114.8 / ‑1.9	45	A488S (GCT→TCT)	yphE	fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (0/43);  total (0/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTA  >  W3110S.gb/2675520‑2675588                                     |                                 gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:639633/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:19867/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:201916/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:233844/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:295335/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:301607/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:393840/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:395742/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:501213/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:616912/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:629597/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1092139/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:683122/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:683128/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:698419/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:7167/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:751484/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:765695/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:780337/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:79735/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:831164/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:15648/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1056611/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1109836/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1170065/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1189644/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1191780/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:120127/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1216186/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1247364/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1289548/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1299214/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1334058/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1357555/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1400340/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1450967/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1464953/62‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:186767/62‑1 (MQ=255)  aaTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAActtgccg         <  1:1113715/61‑1 (MQ=255)  aaTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:617162/61‑1 (MQ=255)  aaTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1466444/61‑1 (MQ=255)  aaTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:912380/61‑1 (MQ=255)   aTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:90468/60‑1 (MQ=255)        ggACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGta  <  1:1246862/62‑1 (MQ=255)        ggACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGta  <  1:434422/62‑1 (MQ=255)                                     |                                 GAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTA  >  W3110S.gb/2675520‑2675588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑