Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,752,892 T→G R21R (CGA→CGC)  yfjF ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,752,8920TG85.9% 420.4 / 42.6 170R21R (CGA→CGC) yfjFhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/0);  new base G (0/146);  total (24/146)
Fisher's exact test for biased strand distribution p-value = 1.00e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

TCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGTACTGCTTCTCAGGTAGCGCAT  >  W3110S.gb/2752833‑2752923
                                                           |                               
tctaGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:172507/57‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1439368/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1258514/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1285783/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1299866/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:13380/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1343705/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:562077/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1358728/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1370226/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:545325/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1443811/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1445743/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1466347/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1471118/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:602928/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:207168/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:761177/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:349464/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:784220/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:370468/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:850624/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:925519/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1052850/60‑1 (MQ=255)
tCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:1067963/60‑1 (MQ=255)
tCCAGCAAGACACTGGCGCGAATAGATTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACt                                 <  1:778870/60‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTATCGCAt  <  1:338631/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:568087/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:437606/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:445036/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:59746/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:455257/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:483666/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:5057/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:578071/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:528106/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:512786/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:665433/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:536359/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:72680/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:99503/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:980587/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:968423/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:947185/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:927868/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:81892/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:797016/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:770808/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:606052/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:718205/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:693420/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:685090/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:6677/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:35622/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:654480/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:648301/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:636237/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:248282/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1189874/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1194300/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1199770/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:118065/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1206919/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:123806/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:113203/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1354761/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1381475/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1410405/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:182616/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:189126/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1118742/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:242709/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:267228/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1109080/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1093290/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1080359/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:3242/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1039483/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1008238/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:38060/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:395225/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1036096/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:102585/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:424207/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:432759/61‑1 (MQ=255)
                              tcAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGATTCTCAGGTAGCGCAt  <  1:1017360/61‑1 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAgg                        >  1:332427/1‑38 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGt                       >  1:825357/1‑39 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGt                       >  1:268984/1‑39 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGt                       >  1:319259/1‑39 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGt                       >  1:937888/1‑39 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGt                       >  1:467927/1‑39 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGt                       >  1:397304/1‑39 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGt                       >  1:428890/1‑39 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1140966/60‑1 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:66873/60‑1 (MQ=255)
                               cAACCGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:220197/60‑1 (MQ=255)
                                   cGTCGCGCCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:709941/56‑1 (MQ=255)
                                         gcCCTCCTGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:1150769/50‑1 (MQ=39)
                                            ctcctGCAACGTCACGCGCTGCAGGTACTGCTTCTCAGGTAGCGCAt  <  1:293174/47‑1 (MQ=38)
                                                           |                               
TCCAGCAAGCCACTGGCGCGAATAGCTTCTTCAACCGTCGCGCCCTCCTGCAGCGTCACTCGCTGCAGGTACTGCTTCTCAGGTAGCGCAT  >  W3110S.gb/2752833‑2752923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑