Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,789,003	C→G	D122E (GAC→GAG)	ygaF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,789,003	0	C	G	100.0%	173.2 / NA	61	D122E (GAC→GAG)	ygaF	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (61/0);  total (61/0)

CAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCG  >  W3110S.gb/2788963‑2789024                                         |                      ctgcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:175674/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAAc                   >  1:282517/3‑45 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTgcgcga            >  1:182358/3‑52 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:572095/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:294164/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:31421/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:335637/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:351752/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:399324/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:406668/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:424051/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:488438/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:50030/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:519182/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:541404/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:554604/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:267432/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:573572/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:586641/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:645109/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:648785/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:65208/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:652445/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:712307/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:801034/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:8124/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:856144/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:865864/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:897796/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:97340/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:980816/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1221485/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1072773/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1095411/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1096170/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1102937/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1148645/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1149085/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1161767/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1184985/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:11964/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1203202/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1010091/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:12261/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1302554/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:234214/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:213324/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:183219/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1460235/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:145281/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1429355/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1321845/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1313412/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:1242922/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcc   >  1:1188968/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcc   >  1:1449128/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcc   >  1:337471/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcc   >  1:1102432/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGGAACCTCGCGAGCGCGAAcca  >  1:1201540/3‑61 (MQ=255) cggcggcgAACGGAATCGATCGCGAGTGGTTAAACGCCGAGAAACTGCGCGAGCGCGAAcca  >  1:1144141/3‑61 (MQ=255) cggcggcgAACGCAATCGATCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAAcca  >  1:494432/3‑61 (MQ=255)                                         |                      CAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCG  >  W3110S.gb/2788963‑2789024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑