Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,801,926 T→C I641I (ATT→ATC)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,801,9260TC100.0% 278.2 / NA 98I641I (ATT→ATC) nrdEribonucleoside‑diphosphate reductase 2, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/0);  total (98/0)

TTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATC  >  W3110S.gb/2801879‑2801941
                                               |               
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGgcg             >  1:491810/1‑52 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAg     >  1:1186681/1‑60 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:351722/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:540720/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:533978/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:514325/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:486349/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:447913/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:424631/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:407619/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:392651/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:367177/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:357528/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:555997/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:349327/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:340100/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:311635/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:284270/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:27873/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:270909/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:263390/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:262271/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:255444/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1019045/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:214975/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:664468/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:993070/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:978686/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:960311/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:959562/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:910255/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:892159/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:858394/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:839564/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:831002/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:801469/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:719778/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:219296/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:66213/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:661078/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:641485/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:63779/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:621039/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:612681/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:603949/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:603510/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:598782/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:579608/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1181160/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1250753/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1248747/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:152099/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1246453/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:123949/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1227901/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1218666/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1217012/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1209880/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1185819/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1246709/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1180623/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1166487/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1124603/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1097964/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1075941/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1067819/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1065077/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1061561/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:104264/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1019454/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1406856/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1457896/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1456186/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1443903/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1432805/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1417728/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1417273/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1410380/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1264946/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1380339/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1378086/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1374712/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1266774/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1270284/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1275303/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1282993/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1299970/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1317650/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1362487/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGat   >  1:1374899/1‑62 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGa    >  1:1134204/1‑61 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGa    >  1:1236237/1‑61 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGa    >  1:769652/1‑61 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGa    >  1:264497/1‑61 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGa    >  1:1318971/1‑61 (MQ=255)
tttATGACTAACGAAAATCTGGCGCTGTATCAGGACCCTTACGAAATCGGCGCAGaaaa      >  1:1026923/1‑59 (MQ=255)
tttATGACTAACGAAAATCTGGCGATGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGa    >  1:949794/1‑61 (MQ=255)
tttATGACTAACGAAAATCT‑GCGCTGTATCAGGACGCTTACGAAATCGGCGCAGAAAAGatc  >  1:724275/1‑62 (MQ=255)
                                               |               
TTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATC  >  W3110S.gb/2801879‑2801941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑