Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,802,325 G→A T56T (ACG→ACA)  nrdF → ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,802,3250GA100.0% 175.0 / NA 63T56T (ACG→ACA) nrdFribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (63/0);  total (63/0)

AACTGACGATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGGC  >  W3110S.gb/2802318‑2802379
       |                                                      
aacTAACAATGCTCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1281337/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCg                         >  1:1206257/1‑39 (MQ=37)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACTCGCTGCAAAATGTTATCGgc  >  1:1076546/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTTCAAAATGTTATCGgc  >  1:152743/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCgg   >  1:1028696/1‑61 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCgg   >  1:218547/1‑61 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCgg   >  1:339423/1‑61 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCgg   >  1:920581/1‑61 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCgg   >  1:1155816/1‑61 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCgg   >  1:12313/1‑61 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCg    >  1:842181/1‑60 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:506809/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:709804/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:678720/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:672392/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:656999/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:528266/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:791924/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:494679/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:359142/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:317307/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:293899/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:719499/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:736118/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:79095/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:210635/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:860768/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:881857/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:894929/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:940976/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:954974/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:975827/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:977884/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:984849/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:993328/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1301488/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1027370/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1029400/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1033943/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1043355/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1055131/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1071374/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1108917/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1113261/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1132830/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1186845/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1215846/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:127483/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1298689/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:262322/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1345351/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1380754/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1383793/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1396003/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1405411/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1424191/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1424508/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:182105/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:188536/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:1018266/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:215757/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:232625/1‑62 (MQ=255)
aacTAACAATGCGCGTTTTTACTCGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGgc  >  1:357601/1‑62 (MQ=255)
       |                                                      
AACTGACGATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGGC  >  W3110S.gb/2802318‑2802379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑