Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,804,902	C→T	F79F (TTC→TTT)	proW →	glycine betaine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,804,902	0	C	T	100.0%	250.3 / NA	89	F79F (TTC→TTT)	proW	glycine betaine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/89);  total (0/89)

GCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCC  >  W3110S.gb/2804854‑2804915                                                 |              gCTCGACAGTTGGTTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1139441/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:529661/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:569789/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:508318/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:503938/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:495242/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:464726/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:896065/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:434978/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:432818/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:432534/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:426469/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:420964/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:408264/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:403058/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:38072/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:361908/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:35934/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:344245/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:287354/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:246662/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:703313/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:836814/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:82498/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:819133/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:791724/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:741198/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:72607/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:725314/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:723273/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:717713/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:561948/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:645088/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:631517/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:62997/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:845335/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:852683/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:605921/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:602266/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:570787/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:233027/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1156946/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1326890/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1310813/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1309565/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1272908/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:124940/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1246049/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1208703/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:90552/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1158048/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1341479/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:917599/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1129994/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1123235/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:969997/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1082830/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1061407/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1056573/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1048971/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:10077/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:146170/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:229586/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:218408/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:204534/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:202344/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:19407/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:191858/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:179040/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:162589/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:155854/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1448055/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1435221/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1421553/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1403179/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1378348/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1373974/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1365387/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1363995/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTCTCGTCCCGTCtttc  <  1:1013237/62‑3 (MQ=255) gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTAGTTACCCATTTTCGTCCCGTCtttc  <  1:863221/62‑3 (MQ=255) gCTCGACAGTTGGGGCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1111348/62‑3 (MQ=255)            gggTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:617817/51‑3 (MQ=255)               tCACTGAAGGGATCGACTGGGTCGTTACCCACTTTCGTCCCGTCtttc  <  1:61635/48‑3 (MQ=38)                     aaGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:870722/42‑3 (MQ=39)                     aaGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:441126/42‑3 (MQ=39)                     aaGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:972177/42‑3 (MQ=39)                      aGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1179718/41‑3 (MQ=39)                      aGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1135060/41‑3 (MQ=39)                                                 |              GCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCC  >  W3110S.gb/2804854‑2804915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑