| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,814,061 | C→T | Q345Q (CAG→CAA) | gshA ← | gamma‑glutamate‑cysteine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,814,061 | 0 | C | T | 81.9% | 181.6 / 29.3 | 105 | Q345Q (CAG→CAA) | gshA | gamma‑glutamate‑cysteine ligase |
| Reads supporting (aligned to +/- strand): ref base C (0/19); new base T (0/86); total (0/105) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAA > W3110S.gb/2814049‑2814116 | gAATCGCACCTGTTGTTCATCTACACCAATCGGCGATAACGGGTTGATGTCCagcgagcgg < 1:1322996/61‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:425976/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:527785/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:500398/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:495931/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:493393/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:487535/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:473960/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:472726/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:456203/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:451416/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:529540/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:418929/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:40347/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:388644/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:377991/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:343257/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:276050/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:264156/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:250030/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:2406/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:725357/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:862789/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:854976/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:845566/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:838573/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:823163/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:810666/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:810448/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:806354/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:775623/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:748017/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1003234/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:721499/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:72034/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:678142/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:677249/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:629448/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:588878/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:564483/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:562081/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:546904/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1170188/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1019414/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1023605/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1024761/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1291751/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1038002/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1250157/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1191279/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1191172/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1189683/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1293122/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1038532/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:105097/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1055865/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1107002/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1084720/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:10775/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1073026/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1068196/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1470441/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:232850/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:198967/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:158654/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:149661/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:147198/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1308261/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1000095/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1461438/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1419514/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1411209/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1397188/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1347769/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1329922/60‑2 (MQ=255) aaTCGCACCTGTTGTTCATCTACACCAATCGGCGACAACGGGTTGATGTCCagcgagcgg < 1:888953/60‑2 (MQ=255) aTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1422773/59‑2 (MQ=255) aTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:1289990/59‑2 (MQ=255) aTCGCACCTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:702056/59‑2 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:804902/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:969276/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:783199/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:878510/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:951754/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:1069166/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:678210/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:1116936/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:659015/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:657547/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:632311/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:1257075/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:498325/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:1303098/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:49094/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:262728/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:255792/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCaa < 1:215077/61‑1 (MQ=255) aCCTGCTGTTCATCTACACCAATCGGCGAGAACGGGGTGATGTCCAGCGAACGCACTTCaa < 1:241724/61‑1 (MQ=255) ccTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:398049/53‑2 (MQ=255) ccTGTTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCagcgagcgg < 1:870333/53‑2 (MQ=255) | GAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAA > W3110S.gb/2814049‑2814116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |