Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,854,208 G→T V405V (GTG→GTT)  fhlA → DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,854,2080GT97.3% 299.0 / ‑0.6 111V405V (GTG→GTT) fhlADNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (108/0);  total (108/3)
Fisher's exact test for biased strand distribution p-value = 4.51e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 111 total aligned reads displayed.

CAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGG  >  W3110S.gb/2854160‑2854228
                                                |                    
cAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTg         <  1:989118/62‑1 (MQ=255)
cAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTg         <  1:1448056/62‑1 (MQ=255)
cAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTg         <  1:43842/62‑1 (MQ=255)
       cctAAAC‑AGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:617995/3‑61 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTc             >  1:715313/1‑51 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGaa       >  1:1291959/1‑57 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGTAACTgg  >  1:1278245/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:552249/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:540130/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:524821/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:518772/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:50834/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:50582/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:308556/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:504625/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:503503/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:563430/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:462673/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:448653/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:431906/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:422596/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:400455/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:390863/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:37486/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:347018/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:342915/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:312938/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:311411/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:3039/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:767218/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:974920/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:907795/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:887060/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:859520/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:856762/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:838151/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:825433/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:802646/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:791552/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:786892/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:784348/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:574892/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:752008/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:709612/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:699046/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:690767/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:673332/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:672718/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:669851/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:653741/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:651642/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:63529/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1128005/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1249776/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1240076/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1236726/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1217010/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:119746/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1184645/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1183702/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1163076/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1159834/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1151584/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1137928/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1031363/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1112243/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1099159/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1091885/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1084599/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1083239/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1078304/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1058046/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1054049/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1038311/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1036338/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1446178/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:307516/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:298975/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:289050/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:28860/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:262596/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:214274/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:21309/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:199003/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:193373/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:191076/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1464205/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1326338/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1415010/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1386672/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1384107/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1371650/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1370907/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1368978/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:13675/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1353512/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTgg  >  1:1344591/1‑62 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTg   >  1:201159/1‑61 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTg   >  1:1255022/1‑61 (MQ=255)
       cTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTg   >  1:1296996/1‑61 (MQ=255)
                                                |                    
CAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGG  >  W3110S.gb/2854160‑2854228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑