Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,859,831	G→A	V30V (GTC→GTT)	ygbI ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,859,831	0	G	A	100.0%	199.8 / NA	73	V30V (GTC→GTT)	ygbI	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/73);  total (0/73)

AATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGAC  >  W3110S.gb/2859773‑2859833                                                           |   aaTCCCGACGAATGGTCATTTGTGACACATTCATTCTGTCCGTTAGTTCATCAATACTAAc  <  1:232739/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:44557/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:568044/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:557353/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:534355/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:517461/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:502429/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:491920/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:47025/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:455427/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:598280/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:445207/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:415497/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:413787/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:411114/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:410169/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1001202/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:388181/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:73599/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:989224/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:984546/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:949212/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:910091/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:899021/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:889071/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:879074/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:820759/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:388896/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:722576/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:720864/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:693658/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:658978/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:63423/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:616363/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:611515/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:354618/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1021334/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1039799/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1061357/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1067136/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1068113/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1073575/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1091435/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1109483/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1119768/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1124129/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1139788/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1294799/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:134868/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1371177/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1393289/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:376646/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1453784/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:318661/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:145930/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1466143/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:179130/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:261074/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:179117/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:175355/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1470363/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1426560/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTCAGTTCAGCAATACTAAc  <  1:931829/61‑1 (MQ=255) aaTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCAGTTAGTTCAGCAATACTAAc  <  1:884922/61‑1 (MQ=255) aaTCCCGACGAATGGGCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:227695/61‑1 (MQ=255) aaTCCCGACGAATGGCCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1163125/61‑1 (MQ=255)  aTCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:209674/60‑1 (MQ=255)           aaTGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:747032/51‑1 (MQ=255)               gTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:612305/47‑1 (MQ=255)                  atatGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:501084/44‑1 (MQ=255)                    atGTGACACATTCATTCTTTCCGTTAGTTCAGCAATACTAAc  <  1:1387581/42‑1 (MQ=38)                     tgtgACACATTCATTCTGTCCGTTAGTTCAGCATTACTAAc  <  1:1127816/41‑1 (MQ=38)                          cacaTTCATTCTGTCCGTTAGTTCAGCAATACTAAc  <  1:1466831/36‑1 (MQ=255)                                                           |   AATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGAC  >  W3110S.gb/2859773‑2859833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑