Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 2,862,422 | G→A | P58P (CCG→CCA) | ygbL → | predicted class II aldolase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,862,422 | 0 | G | A | 99.1% | 298.9 / ‑5.9 | 110 | P58P (CCG→CCA) | ygbL | predicted class II aldolase |
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (0/109); total (0/110) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 110 total aligned reads displayed.
GAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTT > W3110S.gb/2862374‑2862432 | ctaTTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1463998/57‑1 (MQ=255) tACTTGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1135817/54‑1 (MQ=255) tACTGGCGTCACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:97039/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCttt < 1:509070/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:527138/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:661005/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:630879/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:624675/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:619276/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:555413/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:549967/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:547628/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:541334/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:535729/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:349946/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:362835/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:519276/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:354907/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:504982/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:448276/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:441174/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:421543/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:358483/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:388434/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:3842/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:369292/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:807983/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:952900/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:910823/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:892436/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:878211/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:872828/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:866974/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:861614/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:861450/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:844783/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:842029/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:809203/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:66945/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:804504/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:801408/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:781057/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:771750/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:761218/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:760427/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:73811/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:724639/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:712057/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:706909/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:682138/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1238867/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:133944/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1025269/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:132646/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1314477/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1311406/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1307126/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1278229/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:12613/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1261236/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:124593/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1245095/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1337010/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1226890/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:120720/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1183797/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1152396/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1136790/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1125205/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1090911/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1090415/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1050390/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1048736/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1039845/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:219690/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:326634/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:306857/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:292563/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:259669/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:246776/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:243841/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:2286/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:226744/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1342257/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:20558/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:179988/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:159356/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1462741/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1453507/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1452922/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1431166/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:141650/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1405347/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1381640/54‑1 (MQ=255) tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:136311/54‑1 (MQ=255) aCTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:249082/53‑1 (MQ=255) ggCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:1199658/50‑1 (MQ=255) gCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:934448/49‑1 (MQ=255) gACACCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:554044/47‑1 (MQ=255) acCGACAGGTTCATGCCTGGGCAATCTCGATCCACAGCGGCttt < 1:106453/44‑1 (MQ=255) | GAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTT > W3110S.gb/2862374‑2862432 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |