Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,888,219	C→T	E179E (GAG→GAA)	cysI ←	sulfite reductase, beta subunit, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,888,219	0	C	T	100.0%	160.9 / NA	59	E179E (GAG→GAA)	cysI	sulfite reductase, beta subunit, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (59/0);  total (59/0)

CCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTC  >  W3110S.gb/2888170‑2888231                                                  |             ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:716540/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1012676/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:362876/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:42021/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:473826/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:49977/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:544731/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:549178/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:551612/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:581949/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:583036/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:626961/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:670608/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:690761/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:355598/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:717313/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:729222/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:765408/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:778589/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:854791/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:855542/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:871968/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:904074/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:920227/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:932280/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:935451/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:960779/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:978696/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:32776/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1042635/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1058219/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1085221/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1129929/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1149203/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1149799/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:117394/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1202159/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1202612/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1203327/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1230985/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1270271/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1290039/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1346074/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:352777/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:290643/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:257140/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:214535/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:146140/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1458137/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1444022/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1408052/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1400361/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1372354/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAttttc  >  1:1294254/1‑62 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAtttt   >  1:806108/1‑61 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAtttt   >  1:532944/1‑61 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAtttt   >  1:877541/1‑61 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAtttt   >  1:210664/1‑61 (MQ=255) ccTGATCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAGCAGATGTTCAGAAAtttt   >  1:1018996/1‑61 (MQ=255)                                                  |             CCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTC  >  W3110S.gb/2888170‑2888231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑