| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,035,221 | T→C | E514E (GAA→GAG) | recJ ← | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,035,221 | 0 | T | C | 87.5% | 240.5 / 16.5 | 112 | E514E (GAA→GAG) | recJ | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base T (0/14); new base C (0/98); total (0/112) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
CATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGAA > W3110S.gb/3035203‑3035280 | cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:480342/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:602728/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:586765/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:581787/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:554771/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:550043/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:547075/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:542762/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:497498/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:496304/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:494458/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:481021/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:615394/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:447987/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:41784/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:404560/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:393408/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:377562/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:332712/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:305034/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:24437/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:236336/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:227267/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:867757/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:982582/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:980392/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:974503/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:973012/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:972128/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:946976/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:917845/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:899980/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:892552/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:876072/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1005664/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:784883/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:780122/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:777633/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:754969/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:739286/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:730892/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:709213/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:670085/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:670011/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:655834/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1113153/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:134023/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1327324/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1300690/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1255294/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1254349/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1238471/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:22211/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1232535/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1102133/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1347678/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1209142/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1207224/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1117377/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1201078/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:118273/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1168455/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1158968/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1123733/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:106870/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1124/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:205274/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:198040/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:158088/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1002520/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1026411/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:154850/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:147483/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1054282/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1455985/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1449595/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1442993/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1442859/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1360194/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1074124/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:109627/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:1358636/44‑1 (MQ=38)cATCACCTTCAAATGACGCTCGCCCACAAGCCGCTGTTgcaaca < 1:575185/44‑1 (MQ=37) ccTTCAAATGACGCTCGCCCACCAGCCGCTGTTgcaaca < 1:996252/39‑1 (MQ=37) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:1236432/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:801500/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:780248/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:113868/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:1205658/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:1221129/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:617674/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:1358837/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:1465285/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:157925/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:223082/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTACGGGaa < 1:798370/61‑1 (MQ=255) gTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGGCAAACAGCGGCTCCGGGaa < 1:309579/61‑1 (MQ=255) gTTCGCCCACAAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:482689/61‑1 (MQ=255) ttCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:1127405/60‑1 (MQ=255) ttCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGaa < 1:400964/60‑1 (MQ=255) | CATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGAA > W3110S.gb/3035203‑3035280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |