Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,051,620 G→A A185A (GCC→GCT)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,051,6200GA100.0% 363.9 / NA 139A185A (GCC→GCT) ubiH2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/139);  total (0/139)

Only 100 of 139 total aligned reads displayed.

GGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTC  >  W3110S.gb/3051596‑3051631
                        |           
ggATGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:235527/36‑1 (MQ=16)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:368555/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:544428/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:523149/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:510277/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:491945/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:490028/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:462231/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:459191/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:456619/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:449844/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:446998/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:414265/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:401388/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:547981/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:316705/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:313094/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:310151/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:297544/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:287631/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:278959/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:271189/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:245637/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:231291/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1019777/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:724108/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:992285/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:974648/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:892873/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:869344/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:865199/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:850521/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:839205/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:805058/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:804540/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:799359/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:753059/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:748073/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:225204/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:723107/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:717881/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:693098/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:664304/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:60406/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:599809/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:596226/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:581777/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:578272/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:571309/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:553062/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1201008/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:223093/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1292769/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1287973/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1273292/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1268564/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1265907/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1262698/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1246994/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1237293/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:123658/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1211386/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1204784/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1293706/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1165895/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1138252/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:113218/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1102475/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:109575/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1073921/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1073231/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1062767/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1061426/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1056779/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1028930/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1440507/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:21873/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:217723/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:20663/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:204073/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:198/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:191142/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:187295/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:149670/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1472392/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:145687/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1306975/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1435332/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1423729/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:141231/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:140613/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1396104/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1392579/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1389756/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:134585/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1329879/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1309821/36‑1 (MQ=25)
ggAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1307490/36‑1 (MQ=25)
ggAAGTCACAACGTTCGCAATCACAGCCAGTTGTTc  <  1:1112975/36‑1 (MQ=16)
 gAAGTCGCAACGTTCGCAATCACAGCCAGTTGTTc  <  1:289829/35‑1 (MQ=25)
                        |           
GGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTC  >  W3110S.gb/3051596‑3051631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑