Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,154,520	G→C	P170P (CCG→CCC)	yqhD →	alcohol dehydrogenase, NAD(P)‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,154,520	0	G	C	100.0%	88.6 / NA	33	P170P (CCG→CCC)	yqhD	alcohol dehydrogenase, NAD(P)‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (33/0);  total (33/0)

GTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTG  >  W3110S.gb/3154471‑3154532                                                  |             gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGt            >  1:997074/1‑52 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGt   >  1:1309429/1‑61 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:74668/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:361295/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:412442/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:422171/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:514526/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:574661/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:593505/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:615276/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:701300/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:344056/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:824381/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:835178/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:972503/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:974902/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:998090/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:1008313/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:297503/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:262793/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:246833/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:202055/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:196892/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:1464847/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:1443072/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:1361326/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:1236772/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:1163282/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:108432/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCCGTATTTGCCGTg  >  1:107000/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCAATGTTCAGCCCGTATTTGCCGTg  >  1:579773/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCAATGTTCAGCCCGTATTTGCCGTg  >  1:175096/1‑62 (MQ=255) gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCAATGTTCAGCCCGTATTTGCCGTg  >  1:1350028/1‑62 (MQ=255)                                                  |             GTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTG  >  W3110S.gb/3154471‑3154532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑