Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,224,816	C→T	H147Y (CAT→TAT)	ebgC →	cryptic beta‑D‑galactosidase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,224,816	0	C	T	100.0%	53.0 / NA	22	H147Y (CAT→TAT)	ebgC	cryptic beta‑D‑galactosidase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/22);  total (0/22)

AAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGG  >  W3110S.gb/3224776‑3224836                                         |                     aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:535857/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:965602/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:88710/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:843819/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:794255/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:764678/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:658029/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:570855/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:537613/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:1051971/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:432015/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:368042/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:308438/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:195979/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:194861/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:1414864/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:1296079/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:1252812/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:1245118/61‑1 (MQ=255) aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:1072660/61‑1 (MQ=255)  aaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:722109/60‑1 (MQ=255)                           aaGATGGTTATTTCTATAACAAATAACAACTAcgg  <  1:859369/35‑1 (MQ=255)                                         |                     AAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGG  >  W3110S.gb/3224776‑3224836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑