Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,265,587 G→A I99I (ATC→ATT)  tdcB ← catabolic threonine dehydratase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,265,5870GA84.4% 180.4 / 20.0 96I99I (ATC→ATT) tdcBcatabolic threonine dehydratase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/15);  new base A (0/81);  total (0/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAC  >  W3110S.gb/3265578‑3265642
         |                                                       
tttCCCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1310655/62‑3 (MQ=255)
tttCCCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:566092/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:466659/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:556960/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:553638/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:552376/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:54422/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:518713/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:511302/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:484170/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:480991/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:472493/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:205403/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:445402/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:440570/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:436377/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:405743/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:33714/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:333347/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:300417/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:23678/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:234649/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1032748/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:847706/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:970464/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:964459/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:963770/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:9389/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:932927/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:931983/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:918985/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:87282/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:859969/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:852023/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:59263/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:831830/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:823470/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:717255/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:696912/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:663154/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:662035/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:637479/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:606171/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:593641/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1183851/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1329647/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:131043/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1304996/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1291186/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1262509/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1255344/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1247044/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:124417/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1210949/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:169893/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1177659/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1157514/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1117503/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1116987/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:111262/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:109359/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1053482/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1051357/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1032896/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1336073/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:202633/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:165610/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:151383/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1468348/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1465213/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1421898/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1416534/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1411485/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1408522/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1370392/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1331724/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1366105/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1344924/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:1352929/62‑3 (MQ=255)
tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGAATGGTTGCCTGctc     <  1:731505/62‑3 (MQ=255)
 ttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc     <  1:707051/61‑3 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:521792/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:970719/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:1013196/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:164206/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:148230/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:908455/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:901571/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:126353/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:1166409/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:1446845/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:831688/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:79011/61‑1 (MQ=255)
    ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:756880/61‑1 (MQ=255)
      gTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:1470379/59‑1 (MQ=255)
       tCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc  <  1:973091/58‑1 (MQ=255)
         |                                                       
TTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAC  >  W3110S.gb/3265578‑3265642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑