| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,265,611 | G→C | S91S (TCC→TCG) | tdcB ← | catabolic threonine dehydratase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,265,611 | 0 | G | C | 83.8% | 227.2 / 31.0 | 99 | S91S (TCC→TCG) | tdcB | catabolic threonine dehydratase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/16); new base C (0/83); total (0/99) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAC > W3110S.gb/3265578‑3265642 | tttCCCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1310655/62‑3 (MQ=255)tttCCCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:566092/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:918985/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:484170/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:480991/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:472493/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:466659/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:445402/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:440570/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:436377/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:405743/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:518713/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:33714/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:333347/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:300417/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:23678/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:234649/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:205403/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:202633/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:169893/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:931983/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:662035/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:847706/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:831830/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:852023/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:823470/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:859969/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:87282/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:717255/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:696912/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:663154/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:511302/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:637479/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:606171/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:593641/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:59263/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:556960/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:553638/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:552376/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:54422/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:165610/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1177659/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1304996/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:151383/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:964459/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1262509/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1255344/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1247044/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:124417/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1210949/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1183851/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1291186/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:970464/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1157514/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1117503/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1116987/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:111262/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:109359/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1053482/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1051357/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1032896/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1408522/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:932927/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:9389/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1468348/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1465213/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:963770/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1421898/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1416534/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1411485/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1370392/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1366105/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1352929/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1344924/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1336073/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1331724/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1032748/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1329647/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:131043/62‑3 (MQ=255)tttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGAATGGTTGCCTGctc < 1:731505/62‑3 (MQ=255) ttACCGTCAATACCCAGCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:707051/61‑3 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:970719/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:901571/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:908455/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:1013196/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:831688/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:79011/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:756880/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:521792/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:164206/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:148230/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:1446845/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:126353/61‑1 (MQ=255) ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:1166409/61‑1 (MQ=255) gTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:1470379/59‑1 (MQ=255) tCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:973091/58‑1 (MQ=255) gCATCGCGCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:388513/45‑3 (MQ=255) cgcgCAGGAGAGCGAAACCCCTTGCGCATGGTTGCCTGctc < 1:1008582/41‑3 (MQ=38) agagGGAAACCCCTTGCGCATGGTTGCCCGCAGAAc < 1:476553/36‑1 (MQ=255) | TTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAAC > W3110S.gb/3265578‑3265642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |