Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,267,053	A→G	intergenic (‑133/‑182)	tdcA ← / → tdcR	DNA‑binding transcriptional activator/DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,267,053	0	A	G	100.0%	243.5 / NA	85	intergenic (‑133/‑182)	tdcA/tdcR	DNA‑binding transcriptional activator/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (85/0);  total (85/0)

TAAATATCAACAAGTTAAAGTATAAAAATCAGCATAAACCCTGATTTTTGTCAAAAAA  >  W3110S.gb/3267050‑3267107    |                                                       tAAGTATCAACAAGTTAAAGTATGAAAATCAGTATAAACCCTGACTTTTGTCaaaaaa  >  1:507074/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATaaa                      >  1:916781/1‑38 (MQ=37) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATa                        >  1:897848/1‑36 (MQ=37) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTg          >  1:1422636/1‑50 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCagaaaa  >  1:417999/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:417732/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:583231/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:579019/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:548989/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:542488/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:527629/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:481994/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:469178/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:434061/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:58895/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:374293/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:317181/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:311080/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:309611/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:268728/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:267237/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1010545/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:765288/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:993371/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:941926/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:932382/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:922294/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:901831/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:881117/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:837609/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:832104/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:810652/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:588273/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:756434/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:722123/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:719367/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:702513/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:694533/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:645700/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:60641/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:599673/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:247646/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:122678/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1348282/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1319773/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:240803/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1266446/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1248600/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1242628/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1239364/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1238866/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1318518/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1214808/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1170354/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1145086/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1112118/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1108967/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1107990/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1067327/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1032808/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1406138/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:229396/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:170621/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:168067/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1451736/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1448227/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1432517/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:141068/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1348535/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1397091/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1389790/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1388233/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1377007/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1375352/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1366887/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1361393/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1359904/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaaa  >  1:1349912/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaa   >  1:753349/1‑57 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaa   >  1:724209/1‑57 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaa   >  1:1210971/1‑57 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaa   >  1:105495/1‑57 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTTTTGTCaaaaa   >  1:967447/1‑57 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAACCCTGACTATTGTCaaaaaa  >  1:1212887/1‑58 (MQ=255) tAAGTATCAACAAGTTAAAGTATGAAAATCAGCATAAAACCTGACTTTTGTCaaaaaa  >  1:1105791/1‑58 (MQ=38)    |                                                       TAAATATCAACAAGTTAAAGTATAAAAATCAGCATAAACCCTGATTTTTGTCAAAAAA  >  W3110S.gb/3267050‑3267107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑