| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,314,485 | G→T | T462K (ACG→AAG) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,314,485 | 0 | G | T | 90.5% | 47.0 / ‑2.5 | 21 | T462K (ACG→AAG) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base T (19/0); total (21/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGC > W3110S.gb/3314435‑3314495 | tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTTCCTgcgc > 1:777245/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1275386/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:562642/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:439241/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:388522/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:336396/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:326815/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:212069/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1284866/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1013542/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1254028/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1165597/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1154686/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1150684/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1120290/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:109973/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1074694/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGCCTgcgc > 1:1067726/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCTTTGACTgcgc > 1:586645/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCGTTGCCTgcgc > 1:1438744/1‑61 (MQ=255)tCTGCGGCATTACACCGTCGTCTGCAGCAACAACCAGGACTACGATGTCCGTTGCCTgcgc > 1:104941/1‑61 (MQ=255) | TCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGC > W3110S.gb/3314435‑3314495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |