Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,355,794 T→C R436R (CGT→CGC)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,355,7940TC100.0% 386.8 / NA 134R436R (CGT→CGC) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/134);  total (0/134)

Only 100 of 134 total aligned reads displayed.

GCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACC  >  W3110S.gb/3355755‑3355815
                                       |                     
gCCAGGCGAACTGATGGTGATCTACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:571940/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:453594/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:546990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:543352/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:528153/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:514269/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:506347/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:495806/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:494253/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:484199/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:465617/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:465084/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:46391/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:55040/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:445254/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:416212/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:395049/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:383866/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:381841/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:33002/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:30914/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:29733/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1011408/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:263836/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:262891/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:800483/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:991222/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:988187/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:976123/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:965916/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:964171/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:953511/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:896600/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:878972/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:869129/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:863124/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:804285/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:27591/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:757029/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:745160/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:702959/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:700990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:654418/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:640839/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:634371/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:629131/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:622793/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:606421/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1215707/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1291106/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1289459/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1256692/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:245894/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1253400/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1245692/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1242443/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1234076/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1229125/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1076762/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1223769/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1317863/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1211157/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1101908/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1133776/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1185744/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:118184/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1172821/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1169875/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1167990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1155492/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1135441/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1447911/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:242730/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:238817/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:220237/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:215117/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:197092/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1063743/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1459998/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1455878/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1455150/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1444408/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1444348/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1404176/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:14001/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1397914/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:107666/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1377596/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1369474/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1349793/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1349159/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCCCATTCTACACTCGGCAGAAAcc  <  1:364905/61‑1 (MQ=255)
 ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:56641/60‑1 (MQ=255)
 ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1196712/60‑1 (MQ=255)
 ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1382870/60‑1 (MQ=255)
 ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:338231/60‑1 (MQ=255)
          cTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:63718/51‑1 (MQ=255)
              tGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:924871/47‑1 (MQ=255)
              tGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:119350/47‑1 (MQ=255)
                      gACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1063579/39‑1 (MQ=37)
                                       |                     
GCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACC  >  W3110S.gb/3355755‑3355815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑