| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,355,800 | G→A | L438L (CTG→CTA) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,355,800 | 0 | G | A | 88.7% | 322.7 / 21.2 | 151 | L438L (CTG→CTA) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/17); new base A (0/134); total (0/151) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 151 total aligned reads displayed.
GCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGAT > W3110S.gb/3355755‑3355856 | gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGTCAGAAAcc < 1:373756/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:494253/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:495806/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:474950/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:465617/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:46391/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:460290/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:445254/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:416212/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:953511/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:395049/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:383866/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:33002/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:328213/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:29733/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:268576/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:263836/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:262234/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:245894/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:215117/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:964171/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:640839/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:878972/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:869129/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:820730/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:804285/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:757029/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:747251/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:896600/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:702959/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:678188/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:238817/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:634371/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:629131/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:606421/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:565682/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:55040/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:543352/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:539948/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:514269/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:506347/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:117876/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1256692/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1255026/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1245692/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:965916/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1234076/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1229125/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1220842/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1215707/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:988187/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1185744/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1242443/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1172821/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1167990/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:116518/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1135441/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1133776/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1101908/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1076762/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:107060/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1063743/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1011408/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1303878/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:175699/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1459998/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1455878/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1447911/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1444348/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1418007/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:14001/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1397914/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1369474/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1291106/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1317863/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1349159/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1350188/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:983502/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCCCATTCTACACTCGGCAGAAAcc < 1:364905/61‑1 (MQ=255)gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGAGCATTCTACACTCGGCAGAAAcc < 1:613372/61‑1 (MQ=255) ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1382870/60‑1 (MQ=255) ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:56641/60‑1 (MQ=255) ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1196712/60‑1 (MQ=255) aaCTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1451809/53‑1 (MQ=255) cTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:63718/51‑1 (MQ=255) tGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:924871/47‑1 (MQ=255) tGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:119350/47‑1 (MQ=255) tCGACACTCGCAGTTGGCGCATTCTACACTCGGCAGAAAcc < 1:228934/41‑1 (MQ=25) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATATAGAGtggat < 1:549700/62‑1 (MQ=255) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:942369/62‑1 (MQ=255) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:99199/62‑1 (MQ=255) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:711417/62‑1 (MQ=255) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:406064/62‑1 (MQ=255) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:207019/62‑1 (MQ=255) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:185062/62‑1 (MQ=255) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:120376/62‑1 (MQ=255) aTTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:1034158/62‑1 (MQ=255) ttCTGCACTCGGCAGAAACCGATGACGATCTGAAATGCCGCCATCCATATAAAGAGtggat < 1:685518/61‑1 (MQ=255) ttCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:441209/61‑1 (MQ=255) ttCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGtggat < 1:1381914/61‑1 (MQ=255) | GCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGAT > W3110S.gb/3355755‑3355856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |