Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,396,823	G→A	I276I (ATC→ATT)	rng ←	ribonuclease G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,396,823	0	G	A	100.0%	175.2 / NA	63	I276I (ATC→ATT)	rng	ribonuclease G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/63);  total (0/63)

TCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTA  >  W3110S.gb/3396762‑3396873                                                              |                                                   tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:490409/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:987130/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:889230/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:884071/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:769935/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:516194/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:501384/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:362489/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:311506/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:2369/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:197262/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:1445309/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:1102282/1‑62 (MQ=255) tCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTgg                                                    >  1:1110488/1‑62 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTTGCGTCCGGTGTa  <  1:978655/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:603365/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:620573/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:645743/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:650048/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:657417/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:806181/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:657528/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:664028/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:664575/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:688055/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:697528/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:708134/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:749419/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1256097/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:798041/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:595912/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:825908/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:826263/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:828471/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:830506/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:84023/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:874695/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1089660/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:889741/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:900967/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:949256/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:953379/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1068013/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1198430/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1278927/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:129999/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1376373/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1387472/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1407554/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1432159/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1232473/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1457983/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:164681/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:16586/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:183170/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1227942/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1208440/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:303882/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:594694/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:41674/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:547920/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:530845/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1132263/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1193386/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1029758/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:474809/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:414175/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:402809/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:399632/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:379319/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1195082/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:353931/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:343401/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCAGGTGTa  <  1:619982/60‑1 (MQ=255)                                                     cgctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTCGCGTCCGGTGTa  <  1:836363/60‑1 (MQ=255)                                                      gctcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:791667/59‑1 (MQ=255)                                                        tcgctGAATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCGGTGTa  <  1:1365456/57‑1 (MQ=255)                                                              |                                                   TCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTA  >  W3110S.gb/3396762‑3396873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑