Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,401,901 G→A R429R (CGC→CGT)  yhdA ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,401,9010GA100.0% 332.6 / NA 120R429R (CGC→CGT) yhdAconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/120);  total (0/120)

Only 100 of 120 total aligned reads displayed.

ATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACC  >  W3110S.gb/3401871‑3401931
                              |                              
aTTACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:677258/61‑1 (MQ=255)
 ttACCATCGAAGATGTGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:593513/60‑1 (MQ=255)
 ttACCATCGAAGATGCGTCACATTAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:697393/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:453783/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:605195/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:595342/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:591383/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:560613/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:543066/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:519363/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:508531/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:467961/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:615147/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:430820/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:423932/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:422008/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:40505/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:398748/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:384577/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1008465/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:291616/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:283439/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:272731/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:255821/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:815681/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:987958/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:947630/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:932091/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:926990/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:92583/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:893998/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:891080/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:873102/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:865991/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:828624/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:608282/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:808320/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:804777/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:800273/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:75070/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:73539/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:725440/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:659926/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:65818/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:328518/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1120239/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1288865/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:122745/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:230869/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:121040/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1204389/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1195707/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1181358/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1178064/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1163166/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1147813/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1126434/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1211980/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1115648/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1110992/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1099874/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1094247/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1061754/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:106171/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1059571/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1050930/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1041011/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1025659/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:102551/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1303727/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:228494/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:216282/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:210768/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:208451/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:202013/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:161616/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1458604/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1436479/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1391669/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1389332/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1377401/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:130084/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1360071/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1307341/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1354596/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1323385/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGCAAcc  <  1:557286/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCAGGCCTTCGCGAGTAAcc  <  1:527352/60‑1 (MQ=255)
 ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCAG‑CCTTCGCGAGTAAcc  <  1:685004/59‑1 (MQ=255)
 taaCCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:767719/58‑1 (MQ=255)
  tACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:709672/59‑1 (MQ=255)
  tACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:184733/59‑1 (MQ=255)
         gAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:582700/52‑1 (MQ=255)
          aaGATGCGGCAGATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:487425/51‑1 (MQ=255)
            gATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:285857/49‑1 (MQ=255)
              tGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:391876/47‑1 (MQ=255)
               gCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:684808/46‑1 (MQ=255)
                    acaTGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1069395/41‑1 (MQ=255)
                     caTGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:1068397/40‑1 (MQ=255)
                       tGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc  <  1:880269/38‑1 (MQ=37)
                              |                              
ATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACC  >  W3110S.gb/3401871‑3401931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑