| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,401,916 | A→G | G424G (GGT→GGC) | yhdA ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,401,916 | 0 | A | G | 99.2% | 341.2 / ‑4.0 | 120 | coding (1272/1941 nt) | yhdA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (0/119); minor base . (0/1); total (0/120) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
ATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACC > W3110S.gb/3401871‑3401931 | aTTACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:677258/61‑1 (MQ=255) ttACCATCGAAGATGTGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:593513/60‑1 (MQ=255) ttACCATCGAAGATGCGTCACATTAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:697393/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:453783/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:605195/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:595342/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:591383/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:560613/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:543066/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:519363/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:508531/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:467961/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:615147/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:430820/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:423932/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:422008/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:40505/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:398748/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:384577/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1008465/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:291616/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:283439/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:272731/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:255821/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:815681/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:987958/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:947630/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:932091/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:926990/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:92583/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:893998/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:891080/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:873102/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:865991/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:828624/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:608282/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:808320/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:804777/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:800273/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:75070/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:73539/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:725440/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:659926/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:65818/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:328518/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1120239/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1288865/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:122745/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:230869/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:121040/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1204389/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1195707/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1181358/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1178064/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1163166/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1147813/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1126434/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1211980/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1115648/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1110992/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1099874/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1094247/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1061754/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:106171/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1059571/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1050930/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1041011/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1025659/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:102551/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1303727/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:228494/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:216282/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:210768/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:208451/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:202013/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:161616/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1458604/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1436479/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1391669/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1389332/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1377401/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:130084/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1360071/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1307341/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1354596/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1323385/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGCAAcc < 1:557286/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCAGGCCTTCGCGAGTAAcc < 1:527352/60‑1 (MQ=255) ttACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCAG‑CCTTCGCGAGTAAcc < 1:685004/59‑1 (MQ=255) taaCCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:767719/58‑1 (MQ=255) tACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:709672/59‑1 (MQ=255) tACCATCGAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:184733/59‑1 (MQ=255) gAAGATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:582700/52‑1 (MQ=255) aaGATGCGGCAGATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:487425/51‑1 (MQ=255) gATGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:285857/49‑1 (MQ=255) tGCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:391876/47‑1 (MQ=255) gCGGCACATGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:684808/46‑1 (MQ=255) acaTGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1069395/41‑1 (MQ=255) caTGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:1068397/40‑1 (MQ=255) tGAGTTCACGATGATGAACCCGGCCTTCGCGAGTAAcc < 1:880269/38‑1 (MQ=37) | ATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACC > W3110S.gb/3401871‑3401931 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |