| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,495,890 | G→A | E477E (GAG→GAA) | ptsA → | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,495,890 | 0 | G | A | 97.6% | 101.5 / ‑5.5 | 41 | E477E (GAG→GAA) | ptsA | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (40/0); minor base T (1/0); total (41/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAAACCCGTTCCTCGGTTATCGCGCCGTGCGTATTTATGAAGAGTACGCGTCGTTGTTTAC > W3110S.gb/3495846‑3495907 | gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGATTACGCGTCGTTGTTTAc > 1:245817/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTa > 1:275364/4‑61 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTa > 1:233204/4‑61 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:460184/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:27851/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:286893/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:309513/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:348847/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:406793/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:445308/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:454829/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:983213/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:462534/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:545529/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:758426/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:796123/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:886284/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:891489/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:896767/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:920343/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:952585/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1041402/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1416852/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1131247/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1140129/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1141427/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1147042/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1149761/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1275455/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:133672/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1366207/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1369829/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:251524/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1417547/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1437064/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:1452365/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:166347/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:197735/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:237406/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTTAc > 1:24021/4‑62 (MQ=25)gctaaCCCGTTCCTCGGTTATCGCGCGGTGCGTATTTATGAAGAATACGCGTCGTTGTTAAc > 1:188210/4‑62 (MQ=21) | GCAAACCCGTTCCTCGGTTATCGCGCCGTGCGTATTTATGAAGAGTACGCGTCGTTGTTTAC > W3110S.gb/3495846‑3495907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |