Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,528,575 A→G G185G (GGT→GGC)  pfkA ← 6‑phosphofructokinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,528,5750AG82.1% 298.7 / 45.3 162G185G (GGT→GGC) pfkA6‑phosphofructokinase I
Reads supporting (aligned to +/- strand):  ref base A (29/0);  new base G (133/0);  total (162/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

GAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGCAATGGCCGCAGCCAACGTCAGATCTCCACAATAACGGCCCATCACTTCC  >  W3110S.gb/3528539‑3528629
                                    |                                                      
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:569784/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:460311/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:25185/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:1401813/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:820269/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:821766/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:551360/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:849379/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:300712/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:919227/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:1162395/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:1160740/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:1285450/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:1454211/1‑42 (MQ=255)
gAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGc                                                   >  1:720623/1‑42 (MQ=255)
                              acaGCCGCCGGCAGTAGCCGCAGCCTACGTCAGATCGCCACAATAAc                >  1:1308166/1‑47 (MQ=255)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGAtcg                          >  1:913046/1‑36 (MQ=25)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACgg              >  1:1333818/1‑49 (MQ=37)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACgg              >  1:541664/1‑49 (MQ=37)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACg               >  1:1448923/1‑48 (MQ=37)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGcc            >  1:213873/1‑51 (MQ=37)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACttac  >  1:626949/1‑59 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACttac  >  1:363959/1‑59 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:532264/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:664925/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:597175/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:521779/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:506528/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:435952/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:430005/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:41670/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:406756/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:399418/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:394801/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:38777/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:784224/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:982239/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:96027/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:95136/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:941673/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:879374/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:860611/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:83470/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:828399/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:794832/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:616598/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:763908/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:751690/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:750093/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:725631/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:683737/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:335981/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:628650/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:616976/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1264922/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1396722/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:36915/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1327039/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1323269/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1280031/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1279599/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1274679/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:127211/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:126843/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1265398/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1376919/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1251103/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1230018/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1228135/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:121498/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1178082/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1153400/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1130010/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1096318/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1017176/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:197910/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:336789/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1006171/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:317958/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:29066/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:27702/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:265252/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:228578/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:224557/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:219891/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:187754/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:185034/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:181194/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:174691/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1448512/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:1437672/1‑61 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTc   >  1:199757/1‑60 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTc   >  1:779743/1‑60 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTc   >  1:444459/1‑60 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTc   >  1:1305952/1‑60 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTc   >  1:1201413/1‑60 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTc   >  1:70621/1‑60 (MQ=38)
                              acaGCCGCCGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTc   >  1:14083/1‑60 (MQ=38)
                              acaGCCGCCGGCAATAGCCGAAGCCAACGTCAGATCGCCACAATAACGGCCCATc        >  1:644741/1‑55 (MQ=255)
                              acaGCCGACGGCAATAGCCGCAGCCAACGTCAGATCGCCACAATAACGGCCCATCACTTcc  >  1:575281/1‑61 (MQ=255)
                                    |                                                      
GAATTCAACTTCCGGAACCACAACGAATTCACAGCCACCGGCAATGGCCGCAGCCAACGTCAGATCTCCACAATAACGGCCCATCACTTCC  >  W3110S.gb/3528539‑3528629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑