Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,537,835 G→A Y97Y (TAC→TAT)  rhaR ← DNA‑binding transcriptional activator, L‑rhamnose‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,537,8350GA100.0% 424.6 / NA 153Y97Y (TAC→TAT) rhaRDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (153/0);  total (153/0)

Only 100 of 153 total aligned reads displayed.

GATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATGTAAAAGAGATCGCCACG  >  W3110S.gb/3537792‑3537852
                                           |                 
gATCGTTAACTGAAGCGTAGGAGTGTTTATCGTCAGCATGAAAATAAAAGAGATCGCCAcc  >  1:670784/1‑60 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCg       >  1:628263/1‑56 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGcc     >  1:1179839/1‑58 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCCCg  >  1:886384/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCAcc  >  1:458631/1‑60 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCAc   >  1:845371/1‑60 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCAc   >  1:1407306/1‑60 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCAc   >  1:51154/1‑60 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCAc   >  1:48592/1‑60 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCAc   >  1:917030/1‑60 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCAc   >  1:418904/1‑60 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:565897/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:404522/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:634091/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:415165/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:619121/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:609612/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:589744/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:586494/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:576432/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:450699/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:561501/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:543229/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:537797/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:530459/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:658334/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:423824/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:425116/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:477098/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:803158/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:985894/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:985443/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:963996/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:937689/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:932326/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:898596/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:886555/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:869743/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:85278/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:846161/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:648688/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:798691/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:780112/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:758759/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:752416/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:74409/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:725352/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:715144/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:69140/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:39460/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:656440/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:120074/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1385035/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1382346/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1366076/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:134705/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1343360/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1321975/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:131743/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1268137/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1251261/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1213686/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1200887/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1399209/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1179866/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1146047/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1119143/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1115214/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1101668/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1098553/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1067624/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1066869/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1064077/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1036478/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1029413/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:226329/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1004894/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:372192/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:359682/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:328530/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:326785/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:30915/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:29662/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:284614/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:275817/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:273279/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:267966/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:400198/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:220893/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:202783/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:200480/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:181554/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:174666/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:147508/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1443827/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1428947/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1415467/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1415195/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1401588/1‑61 (MQ=255)
gATCGTTAACGGAAGCGTAGGAGTGTTTATAGTCAGCATGAATATAAAAGAGATCGCCACg  >  1:1093561/1‑61 (MQ=255)
                                           |                 
GATCGTTAACGGAAGCGTAGGAGTGTTTATCGTCAGCATGAATGTAAAAGAGATCGCCACG  >  W3110S.gb/3537792‑3537852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑