Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,607,806	G→A	V699V (GTG→GTA)	fadB →	fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,607,806	0	G	A	100.0%	95.5 / NA	36	V699V (GTG→GTA)	fadB	fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/36);  total (0/36)

GTATGAAGTGCCGGAAGGTCTGCGTAATAAAGCGCGTCATAACGAACCGTACTATCCTCCGG  >  W3110S.gb/3607797‑3607858          |                                                     gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:545620/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:36833/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:38376/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:435220/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:445226/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:46330/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:490017/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:510027/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:534823/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:338376/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:554352/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:575504/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:628121/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:631462/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:716778/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:799685/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:821379/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:946101/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:136246/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1092442/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1128127/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1160510/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1262970/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1265719/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1306360/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1359344/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1050090/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1378625/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1410815/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:153495/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:161397/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:179008/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:194652/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:220846/62‑1 (MQ=255) gTATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:313862/62‑1 (MQ=255)  tATGAAGTACCGGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCgg  <  1:1101100/61‑1 (MQ=255)          |                                                     GTATGAAGTGCCGGAAGGTCTGCGTAATAAAGCGCGTCATAACGAACCGTACTATCCTCCGG  >  W3110S.gb/3607797‑3607858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑