Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,627,885	C→A	I88I (ATC→ATA)	rhtB →	neutral amino‑acid efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,627,885	0	C	A	100.0%	123.1 / NA	48	I88I (ATC→ATA)	rhtB	neutral amino‑acid efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (48/0);  total (48/0)

TTTGGCTGGGAATCCAGCAGTGGCGCGCCGCTGGTGCA  >  W3110S.gb/3627872‑3627909              |                         ttttGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:7263/1‑38 (MQ=18) tttGGCTGGGAATACAGCAATTGCGCGCCGCTGGCGCa  >  1:1138684/1‑38 (MQ=21) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGgcgc   >  1:934462/1‑37 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGgcgc   >  1:543704/1‑37 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:798395/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:493361/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:521443/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:546175/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:607735/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:633682/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:653855/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:707278/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:707801/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:753690/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:785672/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:489915/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:809449/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:829133/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:864767/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:870041/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:909713/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:917240/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:931359/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:933872/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:949596/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:152929/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1103883/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1128165/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1195042/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1203530/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1203603/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1273910/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1288584/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1296820/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1429084/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1450372/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:151598/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:1016865/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:21246/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:286723/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:320678/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:342318/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:343555/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:377828/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:379811/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:388867/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:407511/1‑38 (MQ=25) tttGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCa  >  1:462397/1‑38 (MQ=25)              |                         TTTGGCTGGGAATCCAGCAGTGGCGCGCCGCTGGTGCA  >  W3110S.gb/3627872‑3627909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑