Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,639,730 T→C K222R (AAA→AGA)  xerC ← site‑specific tyrosine recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,639,7300TC100.0% 276.3 / NA 97K222R (AAA→AGA) xerCsite‑specific tyrosine recombinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (97/0);  total (97/0)

TTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCT  >  W3110S.gb/3639673‑3639789
                                                         |                                                           
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTctct                                                          >  1:1180011/1‑61 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:613830/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1386837/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1389892/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1442221/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:168666/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:181896/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:434621/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:4744/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:509845/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:512700/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:514393/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:561400/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:314139/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:732042/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:789375/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:829486/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:832617/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:844332/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:849416/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:9313/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:941612/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:954262/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:997762/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1164000/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1200303/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1210931/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:123461/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1238424/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1240596/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1251570/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1099352/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1270336/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:129400/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1296100/1‑62 (MQ=255)
ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTg                                                         >  1:1321311/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGtt                         >  1:1126154/1‑41 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAg            >  1:152131/1‑54 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:71851/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1055515/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:971420/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:709781/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:981021/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:698410/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:695694/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:994506/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:695595/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:664775/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:627912/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:621124/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:947860/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1195723/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:732606/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1187249/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:804456/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:819488/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:822044/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:551272/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:831284/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1151308/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1149639/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:854236/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:902440/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:105888/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:947951/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1062120/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:256082/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1349299/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1349695/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1403115/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1295403/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1448134/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1464887/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:156391/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:175091/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:198016/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:201245/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:206962/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:210901/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:242256/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:609266/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:393780/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:595540/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:590430/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:120813/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1326645/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:512664/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:480359/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:449961/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:276554/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:369240/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:337742/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:1030107/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:299675/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:292071/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt    >  1:283578/1‑62 (MQ=255)
                                                     gCGTCTCTGCACATTACGCGCGGAGATACGCTGGCCCa                            >  1:187748/1‑38 (MQ=38)
                                                     gCGTCTCTGCACATTA‑‑CGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt  >  1:958633/1‑62 (MQ=255)
                                                         |                                                           
TTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCT  >  W3110S.gb/3639673‑3639789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑